1. How much 6X DNA sample buffer do you need to add to 15 ul of DNA to run it ou
ID: 11888 • Letter: 1
Question
1. How much 6X DNA sample buffer do you need to add to 15 ul of DNA to run it out on an agarose gel?2. An antibody to be used for Western blotting needs to be diluted 1:10,000 for optimum results. What volume of the antibody stock solution do you need to add to 80 ml of buffer to make the blotting solution?
3. Tissue culture medium contains 584 mg/liter L-glutamine. Since this amino acid is labile when stored in dilute solutions, it is prepared as a 100X stock solution and then added to the culture medium when needed. How much L-glutamine would you need to weigh out to prepare 100 ml of the stock solution?
4. You count a suspension of lymphocytes and obtain an average cell count of 25 per hemocytometer grid. You wish to plate out 5 x 10e4 cells in a total of 2 ml for a focus forming assay. What volume of cells would you add to what volume of medium to prepare your assay? Show your work!
5. You want to stain cells with neutral red prepared in phosphate buffered saline (PBS). Neutral red is prepared as a 50X stock in distilled water. How much of this stock solution do you need in order to prepare 40 ml of your working solution?
6. You need 200 ml of physiological saline (0.9% NaCl). At your disposal is a 1M (58.44 g/L) stock of NaCl. What volume of the stock solution and how much water should you use to make the 0.9% solution? (Incidentally, what is the molarity of salt in physiological saline solutions like PBS or TBS?)
Explanation / Answer
1) 3 microliters 2) 8 microliters 3) 5.84g 4).20 milliliters or 200 microliters 5)800 microliters 6) 555 microliters
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