To determine if a patient has been exposed to a specific strain of Staphyloccus
ID: 124509 • Letter: T
Question
To determine if a patient has been exposed to a specific strain of Staphyloccus aureus, you perform an agglutination test. You place 0.5mL of the specific strain of S. aureus solution into 5 tubes and then do a serially dilution of the patient's serum (1:2, 1:4, 1:8, 1:16, 1:32) and add 0.5mL of each serum dilution into the tubes. After 30 minutes (which is the appropriate incubation time for this procedure), you observe agglutination in every tube. Which antibody isotype is most likely mediating the agglutination? Why? (1 point) 2. Specifically_describe what the Coombs reagent is and why it is necessary_for some The Coombs reagent is a mixture of C3 complement proteins and antibodies to immunoglobulin's, primarily the anti-lgG antibody. This reagent was used to see if there was an anti-Rh coated RBC's or not. If there are no anti-Rh antibodies then agglutination will not occur and that is how we know that anti-Rh is not present in RBC's 3. You performed a bemacytometer count on your 10mL cell suspension by adding 20 ul of cells to 20uL of trypan blue (1:2 dilution) and your results were as follows: Region 1 25 cells Region 2 35 cells Region 3 32 cells Region 4 28 cells 4 025Explanation / Answer
According to the Chegg guidelines I'll answer the first question when more than one question is posted. Do not give thumbs down.
1)
Yes, the patient was exposed to that particular strain of S. Aureus. We can conclude that because of the agglutination occurs. Agglutination is the result of the antibody present in the patient's serum and bacteria.
IgM is most likely mediating the agglutination because it has one variable and three constant region, one extra constant region replacing the hinge region. This change helps it to recognize the epitopes present on the invading microorganism's surface and this results in agglutination.
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