Question 1 a-e (1pt). Intermediate filaments are responsible for the structural

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Question 1 a-e (1pt). Intermediate filaments are responsible for the structural integrity of the cell. Phosphorylation of intermediate filaments has been shown in many cases to cause their disassembly in the cell. GFAP (Glial Fibrillary Acidic Protein) is common in glial cells (supportive cells of the nervous system). To investigate the role of phosphorylation of GFAP during cell division you radioactively label methionine in interphase and mitotic cells. Next, you purify labeled GFAP from the cells and analyze using 2D gel electrophoresis (interphase, mitosis, and mix of interphase and mitosis samples). You also, treat identical samples with alkaline phosphatase which removes phosphates from the protein and analyze the same way. See the results below Interphase Mitosis Mixture Large MINUS -Alkaline Phosphatase PLUS Alkaline Phosphatase Small Acidic Basic Separation by Charge

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