Translation can be divided into t Initiation The small subunit of the ribosome b

Question

Translation can be divided into t Initiation The small subunit of the ribosome binds at the S' end of mRNA at the which is complimentary to the 3' end of the small subunit. Translation begins ata hree steps 1) Initiation, 2) elongation, and 3) termination codon on the h corresponding anticodon to the start codon carries a chemically mRNA. The modified methionine. RNA wit Once the small subunit, mRNA, and initiator tRNA complex together, the subunit is added and translation can begin. Elongation Once the start codon and corresponding tRNA are in the P site, the second charged tRNA can enter the site. The amino acid from the tRNA in the A site is added to theo the tRNA in the bond is formed between amino acids in A site and P site, the bond is site. Once the broken between the amino acid and tRNA in the P site leaving it empty. The ribosome then moves along the mRNA in the to direction. This positions the empty tRNA in the site and the tRNA containing the growing figure 15.19 in your textbook. Termination Once the ribosome a -chain in the-site. This process is repeated until termination. See e reaches acodon, translation is terminated. Stop codons do not bind to tRNA, but, and uses water to break the bond between the completed polypeptide and the tRNA in the P site via factors. The release factors, bind to the stop codon in the A site are recognized by word Bank: promoter, antiparallel, 5', complementary, stop, c-terminus, one, 3, messenger, release, terminator, DNA, peptidyl, degenerate, more, amino acid, transfer, 3, codon, charged, ribosomal, RNA, peptide, P, protein, RNA polymerase, A, large, aminoacyl-tRNA synthetase, exit, aminoacyl, ribosome- binding sequence (RBS), initiator, polypeptide, hydrolysis, start, E, tRNA, anticodon, ribosome, rRNA some words may be used more than once or not used at all Practice: Take the following sequence from DNA to protein. 3' TACGGTAAGCATCTTGGCATAACCCCAATTS RNA sequence: Amino acid sequence:

Explanation / Answer

Initiation

1] promoter

2] start

3] complementary

4] polypeptide

Elongation --

1] A

2] C-terminus

3] E

4] peptide

5] 5'

6] 3'

7] P

8] P

9] Peptidyle

10] E

Termination--

1] stop

2] release

3] terminator

PRACTICE--

RNA sequence--

5' AUG CCA UUC GUA GAA CCG UAU UGG GGU UAA 3'

Protein sequence-

START Pro Phe Val Glu Pro Tyr Trp Gly STOP

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