16-What is the chemical composition of slime layer or capsule A. Polypeptides B.

Question

16-What is the chemical composition of slime layer or capsule A. Polypeptides B. Polysaccharides C. Fats D. Nucleic acids 17- Which of the following is FALSE about bacteria? A. they lack a defined nucleus surrounded by nuclear membrane B. they lack cellular organelles such as mitochondria and chloroplast their ribosomes are structurally different than higher cells D. they divide by mitosis E. all the sentences above are true 18-To destroy endospores by heating, they must be exposed for 15-20 minutes to steam under 15 PSI (pounds per square inch) of pressure, which generates a final temperature of 121°C. Such conditions are produced by a. b. c. d. a speed vacuum a microwave an oven an autoclave 19-In negative staining what external bacterial structure can be demonstrated A. Capsule B. Nucleoid C. Flagella D. None of these 20-In sporulation the water content of the endospore is A. Reduce by 1.5% B. Increase by 1.5% C. Increase by 10-30% D. Reduce by 10-30% 21-A microscope has a 10x ocular lens and a 10x objective, what is this microscope's total magnification? A. 10x B. 100x C. Ix D. 1000x in certain bacterial cells. 22-Acid- fast is a technique used to detect presence of A. Lipids B. Citric acid C. Mycolic acid D. Cholera 23-What is the counter stain in acid-fast stain? A. Basic fuchsin B. Acid-alcohol C. Methylene blue D. India ink

Explanation / Answer

16.B)Slime layer is composed of polysaccharide.It is present outside cell wall of gram positive and gram negative bacteria and usually thinner than a capsule.

17.D)bacteria divide by binary fission.Only eukaryotes divide by mitosis.

18.D)autoclave-An autoclave is used to sterilise glasswares and containers.autoclaving destroys all microbes and their spores.

19.A)Capsule-Capsule is a structure destroyed by normal staining ,hence negative staining method is used to demonstrate capsule.

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