9. Which of the following techniques is used for visualizing 300 bp fragment PCR

Question

9. Which of the following techniques is used for visualizing 300 bp fragment PCR product? rn Blottini nt with green flu a. Southern b. Fluorescent labeling with green fluorescent protein (GFP) c. Polyhistidine tagging d. Polyacrylamide gel electrophoresis e. Agarose gel electrophoresis 10. You digested a 250 bp gene amplicon with Hhal restriction enzyme. Your digested product is expected to have 91bp, 83bp, 48bp, and 28bp fragments. Which of the following techniques will you use to visualize the digested product? a. b. c. d. e. Southern Blotting Fluorescent labeling with green fluorescent protein (GFP) Polyhistidine tagging Polyacrylamide gel electrophoresis Agarose gel electrophoresis

Explanation / Answer

9) Green fluorescent protein (GFP) is used in fluorescent microscopy. GFP contains 238 amino acid residues and is usually obtained from jelly fish. GFP gives bright green fluorescence when exposed to blue light in the ultraviolet range. Therefore, fluorescent labelling with GFP can be used for visualising 300bp fragment PCR product (option b).

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