12-The organisms are described below. Which of the following is related to BSL-4

Question

12-The organisms are described below. Which of the following is related to BSL-4? biosafety levels (BSL) for micro- A. Agents that can cause diseases that do not respond to antimicrobial therapy or to vaccines in healthy adults B. Agents that pose only moderate risk in healthy adults but not likely to spread throughout the community, and readily treatable C. Agents that can cause disease in healthy adults that can spread very easily throughout the community though can be readily treatable D. Agents not likely to pose a disease risk to healthy adults 13-Which reagent functions as a decolorizer in the Gram stain? A. Crystal violet B. Iodine C. Copper sulfate D. Alcohol 14-What is the mordant used in the Gram stain A. Iodine B. Crystal violet C. Safranin D. Alcohol 15-In the Schaeffer-Fulton method, malachite green is use to stain A. Vegetative portion of the cell B. Capsule C. Endospores D. The fungi 16-What is the chemical composition of slime la capsule yer or A. Polypeptides B. Polysaccharides C. Fats D. Nucleic acids

Explanation / Answer

1) the BSL-4 deals with the organism for which no treatment is available. there is no vaccine or antibiotic for the causative organism. option A.

2) option D. Alcohol acts as decolorizer in the Gram staining. first stain- crystal violet, then treatment with Mordant Iodine followed by destaining with Alcohol and followed by counterstain Safranin.

3) option A. Iodine solution. this Iodine solution makes a complex with Crystal violet stain.

4) option C. Endospore staining method.

5) option B. slime layer or capsule is made up of polysaccharides.

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