* 19%.ai 9:59 PM lab011exp001.doc Mitosis Experiment 1: Observation of Mitosis i
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* 19%.ai 9:59 PM lab011exp001.doc Mitosis Experiment 1: Observation of Mitosis in a Plant Cell In this experiment, you will look at the different stage of mitosis in an onion cell. Remember that mitosis only occupies one to two hours of the cell cycle, whereas interphase can take anywhere from 18-24 hours. Using this information and the data from your experiment, you can estimate the percentage of cells in each stage of the cell cycle. Materials Onion (Alium) Root Tip Digital Slide Images Procedure: Part 1: Calculating Time Spent in Each Cell Cycle Phase 1. The length of the cell cycle in the onion root tip is about 24 hours. Predict how many hours of the 24 hour cell cycle you think each step takes. Record your predictions, along with supporting evidence, in Table 1 2. Examine the onion root tip slide images on the following pages. There are four images, each displaying a different field of view. Pick one of the images, and count the number of cells in each stage. Then count the total number of cells in the image. Record the image you selected and your counts in Table 2 3. Calculate the time spent by a cell in each stage based on the 24 hour cycle using the equation below. Record your data in Table 2: Hours of Stage 24 x Number of Cells in Stage Total Number of Cells Counted Part 2: Identifying Stages of the Cell Cycle 1. Observe the images of the onion root tip. 2. Locate a good example of a cell in each of the following stages: interphase,Explanation / Answer
Answer 1: The appropriate stage of mitosis in a given image is :
A. Interphase
B. Late Telophase
C. Prophase
D. Anaphase
E. Telophase
F. Metaphase
Answer 2: The most of the cells that seen in the onion root tip were in Telophase. Based on this information, I have learned and believe that the cells have spent a lot more than half of their lifespan in this phase.
Answer 3: I was not able to observe the interphase stage of the cell cycle because in this stage the DNA replication takes place and is not observed in any cell.
Answer 4: During the cell growth, it absorbs lots of nutrients required for the replication and division of cell their volume increases at a particular level, however, the cell is unable to get much bigger without the cell bursting. It’s purely related to the division of cell because it helps them to divide.
Answer 5: Mitosis is the idea behind how a cell can divide to form two daughter cells and the process regarding cell division.
Answer 6: If the mitosis is uncontrolled it leads to the formation of an undifferentiated mass of tissue or abnormal daughter cells.
Answer 7: I believe that the time prediction was pretty accurate because I took into consideration the fact that ninety percent lifespan of a cells is spent in Telophase with only about ten percent being spent in the other four remaining stages.
Answer 8: I found metaphase stage is a little bit interesting because it looked like the cell has just bursted. As compared to other stages of cell division they look circular whereas metaphase looks like it has just exploded. This is because of the chromosomes which become attracted to the spindle fibers in the metaphase stage.
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