1. Assignment 1: By using the general equation Nt = No x 2n where Nt is the numb
ID: 133155 • Letter: 1
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1. Assignment 1: By using the general equation Nt = No x 2n where Nt is the number of cells at given time (in minutes) and No is the initial number of cells, and “n” is the number of generations at that point (number of cell division), calculate how many cells you will have in 30, 60, 90, and 120 minutes if your bacteria has a generation time of 30 minutes and you start with 100 cells at 0 time. 2. Assignment 2: Define and explain what a pure culture is? 3. Assignment 3: Explain the difference between the lag and exponential phases in the growth curve. 4. A bacteria grows optimally at 55o C, pH 9.3 and does not grow in the presence of O2. How should this bacteria be categorized with respect of its temperature, pH, and oxygen requirements? 1. Assignment 1: By using the general equation Nt = No x 2n where Nt is the number of cells at given time (in minutes) and No is the initial number of cells, and “n” is the number of generations at that point (number of cell division), calculate how many cells you will have in 30, 60, 90, and 120 minutes if your bacteria has a generation time of 30 minutes and you start with 100 cells at 0 time. 2. Assignment 2: Define and explain what a pure culture is? 3. Assignment 3: Explain the difference between the lag and exponential phases in the growth curve. 4. A bacteria grows optimally at 55o C, pH 9.3 and does not grow in the presence of O2. How should this bacteria be categorized with respect of its temperature, pH, and oxygen requirements? 1. Assignment 1: By using the general equation Nt = No x 2n where Nt is the number of cells at given time (in minutes) and No is the initial number of cells, and “n” is the number of generations at that point (number of cell division), calculate how many cells you will have in 30, 60, 90, and 120 minutes if your bacteria has a generation time of 30 minutes and you start with 100 cells at 0 time. 2. Assignment 2: Define and explain what a pure culture is? 3. Assignment 3: Explain the difference between the lag and exponential phases in the growth curve. 4. A bacteria grows optimally at 55o C, pH 9.3 and does not grow in the presence of O2. How should this bacteria be categorized with respect of its temperature, pH, and oxygen requirements?Explanation / Answer
1) Nt = No x 2n
Generation time is 30 minutes
Number of cells at 30 minutes = 100 × 2(1) = 200
Number of cells at 60 minutes = 100 × 2(2) = 400
Number of cells at 90 minutes = 100 ×2(3) = 600
Number of cells at 120 minutes = 100 × 2(4) = 800
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