d. Look at the results in panel f at right. The mutations indicated on the x-axi

Question

d. Look at the results in panel f at right. The mutations indicated on the x-axis decrease the ability of y-tubulin to bind GTP. The y-axis of the graph shows "fold increase" in the number of microtubules. "Fold" means multiply "Fold increase" means 5 times, 10 times, 20 times more microtubules. What was the effect of the mutation on the number of MTs? Rubric (2): Effect of mutations are described compared to WIT f 15 10 e. The researchers wrote in the discussion section of their paper, "We propose a model in which GTP binding to y-tubulin has a similar function as in ß-tubulin, namely to regulate interaction with tubulin" Based on the results in panel "f" why do you think y- tubulin-GTP is important for microtubule growth and shrinkage? Rubric (2): Link inability of y-tubulin to bind GTP to result you see. 0 -tubulin mutants (can't bind GTP)

Explanation / Answer

d. In the figure, purple, blue, green and yellow bars indicate mutants and brown bar indicte wildtype. In case of mutants, the bar height is less than that of the wildtype. That means that the fold increase in MTs are reduced in mutants. So the mutaions reduces the increase in the number of MT.

e. As discussed in part d., the mutants affects the number of MTs. These tubulin mutants cannot bind GTP. This means that the GTP bound tubulin is neceessary for MTs increase and the lack of GTPbound tubulin leads to decrease in MTs, in terms of both growth and shrinkage.

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