EXTRA CREDIT QUESTION (5 POINTS) Data for the mobility of three different protei

Question

EXTRA CREDIT QUESTION (5 POINTS) Data for the mobility of three different proteins (X, Y, and Z) using both fluorescent recovery after photobleaching (FRAP) are shown in Figure Q11-40A. bleach bleach bleach ? 8 time time ime Figure Q11-40A It is important to remember that in each of these experiments, the results reflect a real physical difference in the way in which these proteins are situated in the plasma membrane. Provide a plausible explanation for the differences observed in proteins X, Y, and Z.

Explanation / Answer

Protein X- the most mobile among all the other protein. As soon the membrane was photobleached protein molecules started to move to the photobleached area.

Protein Y- As we can see from the graph that the photobleached portion remains as such even after much time has passed. So we can conclude that protein Y is covalently attached to the membrane as it cannot move from its position to the photobleached area.

Protein Z- Protein Z can also move to the photobleached area but its mobility is lesser than protein Y. Also, the concentration of protein Y is more than that of protein X as we can see by comparing the area of the curve protein X and Z after recovery time

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