A 10\"3 is performed on a culture of bacteria in order to perform viable plate c

Question

A 10"3 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 73 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! * Any time less than 1 mL is transfered, a dilution is being performed. » Any time more than 1 mL is transfered, a concentration is being performed

Explanation / Answer

As 0.1 mL of finally diluted culture displays 73 colonies, it has 73 bacteria. Therefore 1 mL of the same culture will have 73x10 = 730 bacteria.

Also the dilution is 10-3, so total no. of bacteria in original culture will be 730 x 103 or 7.30 x 105.

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