5) Antibiotics have been very useful in elucidating the steps of protein synthes

Question

5) Antibiotics have been very useful in elucidating the steps of protein synthesis. If you have arn artificial messenger RNA with the sequence 5'AUGU produce the following polypeptide in a cell free, protein synthesizing system: fMet-Phe-Phe- Phe-Phe...In your search for new antibiotics, you find one called putyermycin, which blocks protein synthesis. When you try it with your artificial mRNA you find the following product: fMet-Phe. What step in protein synthesis does the drug inhibit? a) initiation b) elongation c) translocation d) termination e) cannot determine from the above data 6) Which of the following are the same in both prokaryotes and eukaryotes? a) elongation factors b) stop codons c) the use of fMet.tRNA d) AUG initiation codons e) both b and d f) none of the above 7) Is an example of a ribozyme (BEST answer) a) 16S rRNA b) 23S rRNA c) 5S rRNA d) Introns e) tRNA

Explanation / Answer

Please find the answers below:

Answer 5: Choice b (Translation of methionine amino acid refers to the initiation step. Addition of any amino acid further refers to elongation. The chain terminates then after suggesting failure of elongation)

Answer 6: Choice e (The initiation codon is always AUG and stop codons are always represented by either of UAA, UAG or UGA in both prokaryotes and eukaryotes)

Answer 7: Choice b (THE 23S RNA acts as the catalytic unit during translation, hence a ribozyme)

Answer 8: Choice g (both lacI and lacO serve as negative regulators of the lac operon since it is an inducible operon)

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