35. DNA fragments from a gel are transferred to a nitrocellulose paper during th

Question

35. DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. The purpose of transferring the DNA from a gel to a nitrocellulose paper is to A) permanently attach the DNA fragments to a substrate. B) separate the two complementary DNA strands. C) transfer only the DNA that is of interest. D) prepare the DNA for digestion with restriction enzymes. E) separate out the PCRs. 36. The polymerase chain reaction (PCR) has been used to amplify DNA from which of the following? A) fossils B) fetal cells C) viruses D) bacteria E) all of the above 37. Dideoxyribonucleotide chain-termination is a method of A) cloning DNA. B) sequencing DNA. C) digesting DNA. D) synthesizing DNA. E) separating DNA fragments. 38. The following statement(s) is (are) true about Sanger's nucleotides sequencing method. A) It involves in vitro DNA synthesis B) ddNTPs terminates extension of DNA synthesis. C) It involves separation of DNA of single base length difference. D) All of the above statements are true 39. The phenomenon in which RNA molecules in a cell are destroyed if they have a sequence complementary to an introduced double-stranded RNA is called: A) RNA interference. B) RNA obstruction. C) RNA blocking. D) RNA targeting. E) RNA disposal. 40. DNA fingerprinting is based on the fact that: A) number of repeated sequence is unique in each individual. B) complete human genome sequence has been determined. C) criminals have unique gene. D) DNA can be isolated easily E) small amount of tissue is enough for analysis.

Explanation / Answer

35.) A) ( Explanation - DNA is negatively charged while the membrane is positively charged so it easily gets attached to the membrane)

36.) E) All the above ( explanation - as all the above have intact DNA saved so all of them can be used as a template for PCR)

37.) B) (Explanation - Dideoxy dNTP's have 3'H instead of 3'OH in the carbon ring due to which if one deoxy dTNP is added then due to lack of OH no new nucleotide can be added thus terminating the reaction)

38) D ( all are true)

39) A ) RNA interference ( Explanation - First of all, terms mentioned in other options do not exist. RNA interference is a recent discovery which is way of gene knockdown or silencing by complementary dsRNA which is cleaved into small silencer RNAs by cellular machinery)

40) A) (Explanation - These repeat sequences are known as microsatellites of 10-60 bp length and are unique to an individual.)

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