B. (5 pts) To test the purity of your selenocysteine, you pass an aqueous sample

Question

B. (5 pts) To test the purity of your selenocysteine, you pass an aqueous sample through gel filtration column. As expected, most of sample elutes from the column as a single peak with a MW 168 relative to your MW standards. However, you also notice a second smaller peak that elutes with a MW 336. Other than assuming that your sample has some extraneous impurity in it, can you propose another plausible explanation for the material in this second peak? Clearly explain your reasoning for full credit. g that your sample has some atronek eardy. explain your

Explanation / Answer

The second smaller peak may represents the dimer of selenocysteine (dipeptide). The selenocysteine is an amino acid with molecular weight 168. Here small peak elutes with a molecular weight value 336 which is exactly double of 168 and thus, small peak may represent the dimer of selenocysteine.

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