My lab experiment investigates the effect of pH on hexokinase. However, I need t

Question

My lab experiment investigates the effect of pH on hexokinase. However, I need to clarify the following: I used sucrose( sugar) but I got some mixed results. At low ph 4-6 the Co2 collected in the balloons were higher than at 7. I read that fermentation is optimal at a lower pH. However, the optimal pH for hexokinase is 7.5-9.0? and the balloons increased in size in this pH range. How do I explain the increase in size of the balloons at the lower end of the range and then at the higher end? The data was collected after 15 minutes. My lab experiment investigates the effect of pH on hexokinase. However, I need to clarify the following: I used sucrose( sugar) but I got some mixed results. At low ph 4-6 the Co2 collected in the balloons were higher than at 7. I read that fermentation is optimal at a lower pH. However, the optimal pH for hexokinase is 7.5-9.0? and the balloons increased in size in this pH range. How do I explain the increase in size of the balloons at the lower end of the range and then at the higher end? The data was collected after 15 minutes. I used sucrose( sugar) but I got some mixed results. At low ph 4-6 the Co2 collected in the balloons were higher than at 7. I read that fermentation is optimal at a lower pH. However, the optimal pH for hexokinase is 7.5-9.0? and the balloons increased in size in this pH range. How do I explain the increase in size of the balloons at the lower end of the range and then at the higher end? The data was collected after 15 minutes.

Explanation / Answer

The CO2 produced fills the balloon. As CO2 is produced only in fermentation and not when sucrose is subjected to hexokinase, therefore balloon only gets filled when pH is low as fermentation is favorable at that pH range.

Whereas in range 7.5 to 9 when hexokinase is active, fermentation is not favored, which results in no CO2 production and hence, less expansion of balloon as it was in low pH range.

Related Questions

Navigate

• Browse (All)
• Browse M
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.