The Bacterial Growth Curve A culture of E. coli was grown on suitable culture me

Question

The Bacterial Growth Curve

A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below.

Plot the data using Excel and label all axes and clarify the scales used. Indicate all growth phases observed for this E. coli culture.

Part A - The adaptation phase for the E. coli culture lasted

Part B - The exponential phase for the E. coli culture lasted

Part C - Using the graph, interpolate the generation time (Gt) for the E. coli culture. The generation time is:

Part D - Using the formulas, calculate the generation time for the E. coli culture. The generation time for the culture is:

3 hours 2 hours 1 hour 5 hours 4 hours Viable Counts (CFU/mL) 2.00 x 10 2.10 x 10 2.30 x 10 3.25 x 10 6.60 x 10 1.40 x 10 2.92 x 10 6.10 x 10 1.10 x 109 1.23 x 10% 1.30 x 10 Hours 0 4 10

Explanation / Answer

Part d

Doubling time for E.coli is 20 minmtes.So

If generation time of culture is 75 minm,then generation time for E.coli should be . 75/20 min .=3.75 generations of E.coli of one cell

Similarly, 50/20min =2.5 min

40/20 min=2min

20/20min=1 min

62/20min=3.1 min

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