Read Exercises 2-14 and 7-3 in your lab manual, then complete the following item

Question

Read Exercises 2-14 and 7-3 in your lab manual, then complete the following items 1. Define minimum inhibitory concentration (MIC) 2. Pictured below show a Muller Hinton media plate with Staphylococcus spp. (dark color) spre across the surface, with a penicillin disk (vellow color in the center). Draw an arrow to the zone inhibition? Measuremen t of the zone of inhibition? What are the correct units to use to describe llin? the zone of inhibition? According to table 7-4, is this organism susceptible or resistant to penici 3. Define the following. a. Disinfectant: en b. Sanitation: c. Antiseptics:Cermuides used on ar in lwng tissves d. Antibiotics: Using the terms in question 3, choose the term that best fits the situation below. a. Cleaning a bathroom toilet with bleach. DISIHet b. Applying betadine on a forearm before surgery.ntplic c. Using EtOH on your MIC 206 Lab bench. d. Giving your Dog doxycycline for ticks

Explanation / Answer

1. Minimum inhibitory concentration (MIC): MIC is defined as the minimum concentration of an antimicrobial agent which inhibit the visible growth of a microorganism after overnight incubation.

2. Here white portion around penicillin disk is zone of inhibition. Measurement: Measure edge to edge across the zone of inhibition over the center of the penicillin disk (diameter of the white area including penicillin disk).

3. Disinfectant: Disinfectants are antimicrobial agents that are applied to the surface of non-living objects to destroy microorganisms that are living on the objects.

Sanitation: Sanitation generally refers to the provision of facilities and services for the safe disposal of human urine and feces. The word 'sanitation' also refers to the maintenance of hygienic conditions, through services such as garbage collection and wastewater disposal.

Antiseptics: Antiseptics are antimicrobial substances that are applied to living tissue/skin to reduce the possibility of infection, sepsis, or putrefaction.

Antibiotics: An antibiotic also called an antibacterial, is a type of antimicrobial drug used in the treatment and prevention of bacterial infections. Antibiotics transported through the lymphatic system to destroy bacteria within the body while disinfectants destroy microorganisms found on non-living objects.

4. a. Disinfectants

b. Antiseptic

c. Sanitation

d. antibiotic

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