Your Name: 3. (25 points) Troubleshooting a failed experiment. You perform an im

Question

Your Name: 3. (25 points) Troubleshooting a failed experiment. You perform an immumoblot and after color development using secondary antibody conjugated to alkaline phosphatase there are no visible bands present on the membrane. List the possible reasons for this result and detail how you would determine the specific aspect of the technique that failed to work. Assume that sufficient amounts of protein were loaded on the gel. Be reasonabl comprehensive here and do not blame the TAs. ?. ped, "the·well in ffutCcen reading machin to See

Explanation / Answer

Immumoblot is a kind of laboratory test in which is used for analysis of individual protein in the mixture of the protein. It is done through western blotting technique in which antibodies are to be reacted with specific target protein.. this process is done through gel electrophoresis and after this excess antibody is removed and then first antibody is let to react with secondary antibody.

But when there is novisible bands present in the memberane then it means there is a problem with the sample provided

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