stain, mordant, and counterstain? , hd counterstain. For the Gram stain, what re
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stain, mordant, and counterstain? , hd counterstain. For the Gram stain, what reagents act as the primary meal piep is apar of this process). Detr 7. Outline the steps involved in an endospore stain (remember smear prep is a part of this process). Know which reagents act as the primary stain and counterstain. Understand why endospores are d Why is a dilution series often done before making colony counts? (think along the lines of number of 8. difficult to stain. organisms and what you would see on the plate) 9. Be able to recognize a proper streak for isolation and know the purpose of performing this procedure. 10. What is the Kirby-Bauer Disk Diffusion Method? What is meant by a "Zone of Inhibition"? Given a plate, be able to measure this. For what reason(s) might a chemical that is usually thought to be a good antimicrobial show no zone of inhibition? 11. How would you test for the ability of a microbe to produce catalase? 12. What medium is used to test for hemolysis? Why is this an important test in a clini 3. Be able to identify the following on microscope slides: Gram positive bacteria, Gram negative bacteria the three basic shapes of bacteria, endospores, negative stain Euglena, Oscillatoria, Trypanosoma. For the last genus in this list, what type of human sample is viewe diagnose the infection? Use the "Survey of Microorganisms" Handout to view these organisms OfR can view the actual slides on a microscope in the ACE Center (SSB 2201). 4. Be able to identify the following on microscope slides: Aspergillus, Penicillium, Rhizopus, Parameci Know what bacteriophage and viral plaques are; Know how/why they are related: Understand why bacteriophage must be grown in bacteria. Understand what a plasmid is, and be able to briefly explain how it can be inserted into a bacteri what this process is called. d using acidic dyes? What are basic dyes? Be able to explain th nteria Yes, we discussed this!Explanation / Answer
Endospore staining
Principle
A differential staining technique is used to distinguish between the vegetative cells and the endospores.
Steps in staining
Malachite green is water soluble and has a low affinity for cellular material vegetative cells may be decolourized with water. Primary stain used is malachite green
Safranin is then applied to counterstain any cells which have been decolorized.
At the end of the staining process, vegetative cells will be pink, and endospores will be dark green.
Spores are resistant to heat, dessication, chemicals, and radiation.hence difficult to stain
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