Simple shotgun sequencing: You have obtained the following reads from an unknown

Question

Simple shotgun sequencing: You have obtained the following reads from an unknown double-stranded DNA sequence that is known to be 80 bp long. You are guaranteed that overlapping reads will overlap by at least 5 nt.

a. Align the reads into one or more contigs. How many contigs are there? Show your work.

b. What is the sequence of the 80 bp long strand? (Write 5’ -> 3’; please give the sequence of the strand containing more contigs) Show your work.

5’ TCGGCTAAGAGTAGGCCGGGA 3’

5’ TAGTAACCGGCTTCAA 3’

5’ TTGAATAAATCTGTCGTA 3’

5’ AATCTGTCGTAGTAACCGGCT 3’

5’ TATTCAACCCCAAAGGTCTACACTCC 3’

5’ GGCCGGGAGTGTAGACCTTT 3’

5’ GACGCGACATATCGG 3’

5’ GCGACAT 3’

5’ CGACATATCGGCTAAGAGTAGGCC 3’

5’ TACGACAGATTTATTCAACCCCAAAGGT 3’

Explanation / Answer

a)

b)

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