Ecology of Organisms Experiment 1: Effects of pH on Radish Seed Germination Natu

Question

Ecology of Organisms Experiment 1: Effects of pH on Radish Seed Germination Natural sol pH depends on the parent rock material trom which it was formed and processes, such as cimate. Sol pH is a moasure ot the sol Acxdic soils are considered to have a pH of 50 or lower, whereas 100 or above is considered a strong basic or alkaline sol The pH of sol affects the sokbiäity of nutnents in sod water IR therefore also affects the amount of rutrients available for plant uptake. Diflorent nutrionts are available under ditering p of the acidily or alkalinify In this experiment, we will ook at the effect of phH on the gommination and growth rato of radish seeds to determine the range of pH tolerance for the seed Acdc or basac water will be used to stmulate acidity or akalinity in soll aterials Paper Towel Sheets (out to fit into the petri dish Scisson Sunny Location 2mL45% Acetic Acid ewegn Permanent Marker (31 5 cm Petri Dishes 3 pht Test Strips Radish Seed Packet 2 mL 15% Saturated Solum Baking Soda) Solution, Nalico You Must Provide 5 6 8 9

Explanation / Answer

Radish Seed Germinaton Test

Germination Experiment Observing the effects of different pH levels Experiment

We analyzed that germination of radish seeds after watering them with solutions of varying pH levels. For the first sample .we used a milk solution (2% milk and baking soda), which is very basic and had a pH of 10.

For the Second sample we used lemon juice, which is very acidic with a pH of 3. We then conducted a control experiment, using bottled water, with pH of 7

We predict that both the basic and acidic samples will inhibit form of germination, with the more acidic sample to outlast the basic sample.

We also predicted the control to have the most biomass, followed by the acidic sample, then the basic, after the 5 day period.

In addition to this we believe that each solutions will maintain the same pH during this 5 day span. Hypothesis Materials 3 Petri Dishe s30 radish seeds40 mL water40 mL 2% milk 40 mL pure lemon juice

This retains liquid, and speeds up the germination process by keeping the shell of the seed soft. Step 1... Step 2... Step 3... Saturate each dish as follows:dish with 20mL of milk solution. (pH= 10)
Second dish with 20mL of lemon juice. (pH= 3)

Third dish with 20mL of bottled water. (pH=7) Step 4

Appropriate ten seeds into each sample petri dish. Situate the dishes in a well lit area and observe for 5 days. Ensure that each solution maintains the same pH. On the second day, add the remaining 20mL of each solution to their respective petri dishes. Day 1 Day 5 Control Milk solution (ph 9) Lemon Juice

(pH 3) Day 3 Control Control Milk solution (ph 9) Milk solution (ph 9) Lemon Juice
(pH 3) Lemon Juice
(pH 3) DATA Initial Biomass Final Biomass Change Acidic

The data shows that our hypothesis was accurate in some ways and inaccurate in others. The milk and baking soda solution actually decreased in pH levels, and the coinciding sample showed no sign of germination

We were correct, however, in predicting that the acidic sample would show some germination. Although it was shortlived the seed did respond to this solution Lack of sunlight
UV light as opposed to sunlight.

The fat and oils in the milk blocked poors in the seeds coating, stopping germination.
Skim milk instead of 2%?

Bacterial colonies that formed as a result of the milk.
Bacteria would ultimately deprive the seed of any nutrient or minerals it could derive from milk.


Temperature
Too cold Upon measuring the overall biomass in each sample, apart from the control, the acidic sample was the only to increase reflecting that som germination did infact occur. Conditions Seeds can only germinate and sustain growth
with a solution that has a pH between 5 and 8. Radish seeds typically germinate in 3/4 days

Results

1.Absolute Growth

2.Qualitative Observations

3.8 peat pellets in trays

4.Diluted vinegar and saturated baking soda solutions

5.4 solutions:
pH 9, basic
pH 7, neutral (control)
pH 4, acid rain
ph 2, very acidic
2 plants per treatment
8 hours of sunlight

6.constant cool temperature

7.Watered a few mL every day

8.Recorded for germination

9Monitored and recorded plant growth every 2 days

10.Healthy radish after 24 days


Two leaves, two more leaf buds started branching during the last two days.
Continuous growth

Grew to certain height, and slowed growth while leaves increased
Roots
Radish
Very thin taproot growth

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