E) eloctron Tragment wing a prole, what wast be donr double helis. C) The probe
ID: 136075 • Letter: E
Question
E) eloctron Tragment wing a prole, what wast be donr double helis. C) The probe must be hybeidized with the fragme D) Only A and B are comect. ) A, B, and C are corrext 31. DNA fragments from a gel are transferred to a mitrcelone paper dring procedure called Seuthera Mottiag. The purpone ef wrasfarriag permanently attach the DSA fragments to a subtane ) separate the two C) transfer enly the DNA that is of interow D) prepare the DNA for digestion with restriction enryme E) separate out the PCRs 32. After being digested with a restriction enrymegmie DNA fragmnts are separated by gel electrophoresis.Specific fragments can thes be idetitied through the use of a: A) plasmid B) restriction enzyme. sticky end hucleic acid probe. ) RFLP 3x. Which of these is nor one of the precedures wsed in Southers Motting A) electrophoresis C) autoradiogaphy D) restriction fragment preparation E) mRNA is transferred to nitrocellulose membrane 34. The unpaired aucleotides prodaced by the action of rtrictin auymes are referred to A) sticky ends. B) base sequences. C) single strands D) restriction fragments. E) ligases.Explanation / Answer
Ans
33) C
autoradiography is not a step of southern blotting.
34) A
Sticky ends are produced by action of restriction endonucleases.
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