the DNA procedure talled Souvers B) fetal ce Dj bacteria 1) all of the above A)c
ID: 136076 • Letter: T
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the DNA procedure talled Souvers B) fetal ce Dj bacteria 1) all of the above A)ckerng DNA. C) dipasting DNA D) synthosizing DNA ) separating DNA fagmonts method. A)it involves in vitho DNA synthesis B) JANTPs terminaties extension of DNA syahesis C) E involves separation of DNA o single base lomgh difinmor D) All of he ahove statements are e 19. The phenomeeon in which RNA molecaules in a cell are destred i they have B) RNA obstruction. C) RNA blocking. D) RNA targeting E) RNA disposal. 40. DNA fingerprinting is based on the fact that: A) number of repeated sequence is unique in each indivi B) complete human genome sequence has been determined C) criminals have unique gene. D) DNA can be isolated easily E) small amount of tissue is enough for analysisExplanation / Answer
Answer :
Q.35) A) Permanently attach the DNA segment to a substrate.
This is done just to oversimplify the procedure of southern blotting. The DNA fragments are transferred from agarose gel to nitrocellulose membrane being positive in nature and having high binding capacity for negatively charged DNA fragments so as to perform the further procedure of hybridisation with probe effectively. This is basically done because the process if performed with DNA fragments attached onto the nitrocellulose membrane gives more accurate result of southern blotting than with agarose gel and it is also easier.
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