Next, how much of each stock (listed below) plus water do you use for 500 mL of

ID: 136330 • Letter: N

Question

Next, how much of each stock (listed below) plus water do you use for 500 mL of the buffer recipe above?

The Buffer Recipe is:

150 mM NaCl (formula weight 58.44 g)

10 mM Tris pH 8.0 (formula weight 121 g)

2 mM EDTA (formula weight 292 g)

0.2% bovine serum albumin

Choose units so that your answer is measurable, e.g. not 0.05 L or 50,000 uL, but 50 mL. Show your work and include units in your calculations.

-3 M NaCl ________ ?

-1 M Tris, pH 8.0 ________ ?

-0.5 M EDTA ________ ?

-10% bovine serum albumine ________ ?

-distilled water ________ ?

-3 M NaCl ___50mL_____ ?

150=3*V

V=50mL

-1 M Tris, pH 8.0 ___10mL_____ ?

10mM=1M * V

V=10mL

-0.5 M EDTA ____4mL____ ?

2mM = 0.5 * V

V=4mL

-10% bovine serum albumine ___50mL_ ?

-distilled water __386mL_ ?

500-114= 386mL

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.