used. rag the terms on the left to the appropriate blanks on the right to comple
ID: 140206 • Letter: U
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used. rag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be Reset Help For the footprint protection assay, the DNA tragment is first labeled at one end with one sa5 Two samples of the labeled DNA are then prepared one sample is mixed with TFs and one is treated identically except without the TFs treated with DNase I and analyzed by gel electrophoresis under conditions that resolve DNA fragments that differ in ) Both DNA samples are then two absent by nucleotide(s) experimental sample Each site that can be cut by DNase I will be indicated by a band in the lane corresponding to the control sample If the TFs bind to DNase I from cutting the DNA at those sequences, and the bands corresponding to DNase I cuts at those sequences (the GC box, the CAAT box, and the TATA box) will be three conserved eukaryotic promoter element(s), they will prevent weight length present control sample DNase lExplanation / Answer
For the footprint protection assay, the DNA fragment is first labelled at one end with P32. Two samples of the labelled DNA are then prepared. One sample is mixed with TFs (experimental sample) and one is treated identically except without the TFs (control sample). Both DNA samples are then treated with DNase I and analyzed by gel electrophoresis under condition that resolve DNA fragment that differ in length by one nucleotide.
Each site that can be cut by DNase I will be indicated by a band in the lane corresponding to the control sample. If the TFs bind to three conserved eukaryotic promoter elements, they will prevent DNase I from cutting the DNA at those sequences and the bands corresponding to DNase I cuts at those sequences (the GC box, the CAAT box and the TATA box will be absent.
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