3. To determine the genetic distance between the genes in the above conjugation,

Question

3. To determine the genetic distance between the genes in the above conjugation, you then perform a recombination experiment by mixing the same original Hfr and F- strains. What medium would you select the conjugates on before screening for recombinants to map the genes?

A) Medium lacking pro and containing streptomycin
B) Medium containing pro and lacking streptomycin
C) Medium containing leu and containing streptomycin

D) Medium lacking leu and containing streptomycin
E) Medium containing ser and containing streptomycin

Questions 2-4. Below are the results from the interrupted mating experiment from conjugation between Hfr pro ser* leu Strs X F- pro ser leu Strk showing the number of bacterial colonies found on the different media conditions at different time points. Media Condition MM +Str +pro +leu MM +Str +ser MM +Str +pro +leu Time(min) +ser 20 40 60 40 85 40 85 140 30 90 135 2. Based on the data above from the interrupted mating, what is the order of the genes on the bacterial chromosome in reference to the fertility factor origin (O) B) O leu+ Sert ser+ D) Either A or B E) Either B or C

Explanation / Answer

Q 2-4

In first media condition fertility factor origin O is 20 min. away from ser

In 2nd media condition O is 20 min. away from leu

In 3rd media condition O is 40 min. away from pro

so the order of gene will be

O_ser+_leu+_pro+      OR     O_leu+_ser+_pro+

Answer is D) Either A or B

Q 3 The genetic distance between the genes in the above conjugation, a recombination experiment by mixing the same original Hfr and F- strains

A) Medium lacking pro and containing streptomycin

Streptomycin will ensure that only F-strain survive. In absence of pro only those F- strain will grow which have undergone complete conjugation that means all the genes( ser+ leu+ & pro+) from Hfr strain has entered F- strain.

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