!) Fisher Seientific MATERIALS CHECKIST QUESTIONS (NOTE You may have to do some

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!) Fisher Seientific MATERIALS CHECKIST QUESTIONS (NOTE You may have to do some Internet searching for some off hese answersl) and acetone are both polar solvents This means they have a slight charpe, so anything elue that hasa ponsitive or negative charge on any part of the molecule will more readily dissolve in them land, in chromatography, travel further along the paper strip) If a molecule very close to the origin s non-polar (not charged) it will not dissolve in either of these solvents, and so will remain at or 1. Acetone is much less polar than water. Did this make a difference in the chromatograms (resalts)fo the markers that you tested? Why do you think this may be the case? 2. Some of the markers that you used were water-soluble (and so easier to clean upl). Others were permanent markers. Did this make a difference in how they behaved in each of the solvents? What would that mean for cleaning up? 3. Could this techniques be used in forensics-at a crime scene, in analysis of a historical document, or other investigation? How might it be used? Internet or other sources used to answer the questions:

Explanation / Answer

1) In the process of chromatography, it is necessary to use a solvent that can dissolve the pigment in question. Water being a polar substance, is poor at dissolving polar molecules. Greater the distance the solvent travels in the chromatographic strips, higher is the resolution. Water travels only for a shorter distance than acetone and therefore has lower resolution. Because of these reasons, acetone is preferred over water for chromatography.

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