Your Turn Write-2-20 \", \"20-200 \", or . 100-1,000 \"to indicate which micropi

Question

Your Turn Write-2-20 ", "20-200 ", or . 100-1,000 "to indicate which micropipettor should be used to measure each of the following volumes: 50 20-200ML 0.015 mL 0.6 mL 1.0 mL 0.05 mL Assume that the following measuring devices are available to you: 50 mL beaker 50 mL graduated cylinder 25 mL graduated cylinder 10 mL pipette 5 mL pipette 100-1,000 L micropipettor 20-200 L micropipettor 2-20 L micropipettor Fill in the name of the device that will most accurately measure each of the following volumes: 0.25 mL 2.5 mL 21 mL 0.007 mL 0.8 mL 37 mL 0.08 mL 8.5 mL 0.016 mL 0.04 mL

Explanation / Answer

Pipettes are tool used in the laboratory to accurately measure volumes of liquids. There are different pipettes with different levels of accuracy and precision available. Air disposable micropipettes ranges from sizes of 0.1 ml to 1000 ml. Disposable tips are available that can be used with these pipettes to draw out the liquids.

1. A 2-20 microliter pipette will draw liquid between 2 mlcroliter and 20 microliter. A 2-200 microliter measures liquids between 2 microliter and 200 microliter. A 100-1,000 microliter measures liquids between 100 microliter and 1000 microliter.

50 microliter: 2-200 microliter

300 microliter: 100-1000 microliter

0.015 mL: 2-20 microliter (as 1 mL= 1000 microliter, so 0.15 mL=15 microliter)

0.6 mL: 100-1000 microliter (as 0.6 mL= 600 microliter)

1 mL: 100-1000 microliter (as 1 ml= 1000 microliter)

0.05 mL: 2-200 microliter ( as 0.05 mL= 50 microliter)

2. Similar to pipettes, graduated beakers and graduated cylinders can be also used to measure volumes of liquids. These beakers and cylinders have marking to indicate volumes. A 50 mL beaker can measure and accommodate volume of 50 mL and are labeled from 10 mL-50 mL. Similarly, a 25 and 50 mL graduated cylinders are used to measure 25 and 50 mL volumes. Glass pipettes are also available that measure liquids. These range from 1 mL, 5 mL, 10 mL, 25 mL, 50 mL etc. A rubber bulb is usually attached to suck or draw out the liquid in the pipette. Micropipettes are usually used to measure smaller amounts of liquids in microliters with accuracy and precision.

i) 0.25 mL: 100-1000 microliter micropipettor (as 0.25 mL= 250 microliter).

1 mL pipette can also measure it, but it is not as accurate.

ii) 37 mL: 50 mL graduated cylinder. 50 mL beaker will not have a marking for 37 mL accurate volume.

iii) 2.5 mL: 5 mL pipette

5 mL and 10 mL pipette can measure it as both have markings for 2.5 mL. However, most accurate is 5 mL pipette.

iv) 0.08 mL: 20-200 microliter (as 0.08 ml= 80 microliter)

1 mL pipette can also measure it, but is not as accurate.

v) 21 mL: 25 mL graduated cylinder

Both 25 and 50 mL graduated cylinder can measure this volume. However, 25 mL graduated cylinder is most accurate.

vi) 8.5 mL: 10 mL pipette

25 mL graduated cylinder can also measure this volume. However, pipettes are more accurate than graduated cylinder.

vii) 0.007 ml: 2-20 microliter micropipettor (as 0.007 mL= 7 microliter)

Viii) 0.016 mL= 2-20 microliter micropipettor (as 0.016 mL= 16 microliter)

ix) 0.8 mL: 100-1000 microliter micropipettor (as 0.8 ml= 800 microliter)

1 mL pipette can also measure it, but is not as accurate

x) 0.04 mL= 2-200 microliter micropipettor (as 0.04 ml= 40 microliter)

Please use appropriate symbol for microliter.

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