Information WC1 is agune that has been implicaed in cancar Altermative splicing

Question

Information WC1 is agune that has been implicaed in cancar Altermative splicing of wCt nesults in the praduction at two pratains from the WC1 gone, -KTS and-KTS The figure below shoNs a DNA binding nity assay In a DINIA binding assay an elsctric feid is applied to a gal loaded with DNA and protain Smaler moiecuies will travl uterouig the gal than larger molocuias +PS is a short sequence of DNA that WCt protains typicalily bind to. -P5 was loaded into lane 1 of the gal by itselt (no protain. Lane 2 , ioaded with ony the KTS protans ino DNAL Lars 3 was loaded with both +P5 and-KTS. Lan 4 was oded with both +PS AND Direction of Migration +P5 What does this gure tall us about the binding of wC1 protsins (-KTS and "KITS) to P5 DNA

Explanation / Answer

Gel electrophoresis is a biological technique used to separate macromolecules, in a gel a molecule can sepeeate based upon their sizes. When the sample is loaded in the well, the RNA molecules will start migrating from the well in the gel. In the fig B and D mutated RNA travelled a longer distance in B when compared with D, hence it means that RNA molecules presented in B is smaller ones and in D are larger ones. So according to the sizes it gets separated in the gels B and D. Whereas in the gel C, the molecules are very larger that it couldn’t able to move on in the gel.

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