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conduct your own search and find one story from a major news outlet that concern

ID: 140915 • Letter: C

Question

conduct your own search and find one story from a major news outlet that concerns biology in some way and answer the following

Write the title, dateof publication, and sourceof the article and provide the linkto the article. b)In your own words, summarize the main point of the article and explain how it relates to biology. c)Was the information presented in the article obtained through the scientific method, is it an anecdote, or is it not possible to tell? Justify your answer by referring to the steps of the scientific method described on the first two pages of this handout.For example, what is the hypothesis? Was an experiment conducted, and did the data support or refute the hypothesis? On the other hand, if it’s not clear that the steps of the scientific method were followed, then explain.

Explanation / Answer

Research Article title: Reversible Anti-Fertility Effects of Aqueous Leaf Extract of Ocimum Sanctum (Linn.) in Male Mice.

Dateof publication: JULY-2016

Access this article online : www.ijlssr.com

Sourceof the article: All the data presented in this article is correct because as a research scholar, i have worked on this. This is a research article.

Present study was undertaken to evaluate the reversible anti-fertility effect of Ocimum sanctum Linn
(Tulsi) on male mice. Aqueous leaf extract of Ocimum sanctum was orally administered (0.1ml) for 10 (P < 0.05), 20 (P < 0.01), 30 (P < 0.001), 40 (P < 0.001) and 50 (P < 0.001) days of exposure. Treatment of aqueous leaf extract of Ocimum sanctum showed significant decrease in sperm counts during 10 (P<0.1), 20 (P<0.01), 30 (P<0.01), 40 (P<0.001) and 50 (P<0.001) days treatment than the control. Motility of spermatozoa also declined significantly in treated group after 10 (P<0.01), 20 (P<0.01), 30 (P<0.001) 40 (P<0.001) and 50 (P<0.001) days treatment than the control. However, abnormality of spermatozoa increased significantly in treated group of mice during 10 (P<0.01), 20 (P<0.01), 30 (P<0.001) 40 (P<0.001) and 50 (P<0.001) days treatment than the control. Thus significant decrease in sperm counts, motility and increased abnormality of spermatozoa altered the seminal quality which caused infertility among treated group of mice. Effect of the treatment on reproductive organs and fertility was also investigated. The results revealed that Ocimum treatment caused decrease in the weight of reproductive organs viz., testis (P<0.001), epididymis (P<0.01), seminal vesicle (P<0.001), vas deferens (P<0.01) and ventral prostate (P<0.001) significantly when compared to the control group. The recovery group of animal, which also received 50 days treatment, was maintained for 90 days without any treatment to check the reversibility. All the animals after the recovery period showed normal fertility rate. Thus Ocimum sanctum adversely affects fertility in mice and showed anti-fertility effect among them. From this we can conclude that Ocimum sanctum can be used as a potent anti-fertility agent which is reversible. Key-words- Ocimum sanctum, Anti-fertility, Sperm count, Motility, Sperm abnormality.

Sperm count, Motility, Sperm abnormality, are all related to Biology. In this article, all the data presented, is correct and reliable. I have worked on it and I have used the scientific methods which are as follows: Sperm counts were done by using methods described by Eliasson (1975), motility of spermatozoa was observed after the method of Tijee and Oentoeng (1968) and the morphology of stained sperms was observed under high magnification of the microscope. Abnormalities if present were noted. If 70 % sperms had head, tail and mid-piece the animals were considered to have normal sperms (Sood, 1987). The weights of the dissected organs were calculated for 100g body weight of animal by using the following formula:

Weight of organ = Wet weight of organ/Body weight × 100
= g/1000g b wt. Body weight

Hypothesis: As tulsi has its anti-zygotic, anti-implantational and aboritifacient activity in women and in the experimental animals. Ocimum sanctum leaves show anti-fertility property in male mouse as it possesses anti-spermatogenic. Benzene, extract of Ocimum sanctum in albino rats also decreases the total sperm counts and sperm motility which leads to infertility among them.

From the above said medicinal property, the present study has been undertaken to observe the effect of Ocimum sanctum on Swiss albino male mice to see the reversible anti-fertility effect in male.