The following strains were conjugated: Tet s arg - ara - zeo s lys - str s gal -

Question

The following strains were conjugated:

Tets arg- ara- zeos lys- strs gal- trp- phe- vans lac- kanR x TetR arg+ara+zeoR lys+ strR gal+ trp+ phe+ vanR lac+ kanS (donor strain)

Can someone please explain how you should approach this problem and explain the results.

b. (10 points) Your lab mate is trying to predict what results they will get from their interrupted mating experiment between Hfrl and the recipient strain, and comes to you for help. Based on the genetic map above, predict whether growth would or wouldn't occur on each of the following plates at the indicated time points (assume that each of the plates is minimal media with kanamycin and the supplements indicated). You can indicate that growth would occur by placing a + in the table and indicate that growth would not occur by placing a - in the table. Phenylalanine Argenine Phenylalanine Phenylalanine Tryptophan Phenylalanine Argenine Phenylalanine Tryptophan Argenine T eArgenine Argenine Argenine Argenine Lysine Lysine Lysine VancomyCin Lactose (as only carbon Lysine Tryptophan Lysine vancomycin Thyptophan Tryptophan |Galactose (as Streptomycin only carbon Galactose (as only carbon Source) source) source) 0' 10' 20' 30' 40' 50'

Explanation / Answer

In the genetic map, we can see that Hfr3 direction is anticlockwise. Therefore, first tetracycline gene will transfer to the recipient. Now, the direction will follows and other genes will transfer. In Hfr 1 region, we would get phenylalanine at time 10’ while at the same time we would get Zeomycin gene from Hfr 2.

But here we need to observe Hfr1 gene transfer.

Therefore, we would get phenylalanine at time 10’.

Until time 20’ we get Vancomycin gene.

Moreover, until time 50’ we will not get any gene. After 50’ we get lac gene.

Therefore, we would get only two genes in transfer.

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