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Intermediate: A B C D Amino Acid E Strain 1 + + - + + Strain 2 + + - - + Strain

ID: 14240 • Letter: I

Question

 Intermediate:   A           B            C             D           Amino Acid E

Strain 1              +           +             -             +                   +

Strain 2              +           +             -             -                    +

Strain 3              -            -             -             -                    +

Strain 4              -            +             -             -                    +

+ indicates growth           - indicated no growth

Based on this information, construct the biochemical pathway for E synthesis.  Show the order of the intermediates and the step that is blocked in each mutant strain

Explanation / Answer

From this chart, we can clearly see that Strain 1 requires everything but intermediate C; thus, intermediate C must come first in the biochemical pathway. We can also see that Strain 4 requires no other medium but B; therefore, B is the last intermediate before Amino Acid E. From Strain 2, it is apparent that A and B are subsequent, as are C and D. From this gathered group of information, you can deduce that the biochemical pathway for Amino Acid E is as follows: C --> D --> A --> B --> Amino Acid E Strain 1 is blocked at the production of D Strain 2 is blocked at the production of A Strain 3 is blocked at the production of C Strain 4 is blocked at the production of B Hope this helps!

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