End of Lab Questions 1) You place a new slide on the stage of your microscope an
ID: 142879 • Letter: E
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End of Lab Questions 1) You place a new slide on the stage of your microscope and look through the eyepieces Unfortunately even though the microscope is in focus you still don't see the specimen Describe how you would use the stage control knob and focus knobs to bring the specimen into focus. Tho 2) You have a specimen in focus with the 4X objective and you want to change to the 40X objective to view the specimen at a higher magnification. Explain objective and keep the specimen in focus while increasing the magnification. Keep it simple! how you would switch the 3) You are looking at a specimen but there is not enough contrast to see it clearly. How would you go about increasing the contrast? Again, keep it simple! 4) Define in your own words, contrast and differential staining.Explanation / Answer
Q.1
when we are looking through the ocular lens we have to turn the coarse focus knob carefully and then we have to move slowly the stage away from the lens to bring the specimen into focus. after that, we have to use the fine focus knob for the sharp focusing of the specimen. for sharp focusing visualization of a specimen was done under oil emmersion. by placing a drop of oil on the slide and ensure the there will be the proper immersion of the objective in the oil. after that with the help of fine-adjustment knob to bring the specimen in sharp focus.
Q.2
when we are focusing our specimen we always start with 4X or maybe 10X objective. so after the specimen comes under focus then we can switch our objective to the higher power (40X) . after switching it to the higher power we have to again re-focus our specimen. and while focusing on higher power objective we have to use only fine focus control.
Q.3
for increasing the contrast in a bright light microscope we have to move the condenser to keep it as close as possible to the stage. then all the way we have to close the aperture. then while looking at the eyepiece and then we can check the contrast.
Q.4
Differential staining is defined as when we are using more than one chemical stain or using multiple stains which helps us in differentiating in a better way between different cellular component and structure of a particular organism.
Contrast staining defines as the dye used to color a particular portion of a cell or tissue which remain unaffected during the staining of other parts by different dyes.
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