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1. 2. 3. What are some of the limitations of PCR? Give three important character

ID: 143121 • Letter: 1

Question

1. 2. 3. What are some of the limitations of PCR? Give three important characteristics of cloning vectors. Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths and weaknesses of each method 4 Briefly explain how an antibiotic-resistance gone and the lacz gene can be used to determine which cells contain a particular plasmid 5. Compare and contrast the use of PCR and gene cloning for amplifying DNA fragments. What are the advantages and disadvantages of each method?

Explanation / Answer

Ans.1 : Limitations of PCR

Ans.2 : Characterstics of cloning vectors

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