2. af cells at a concentration of 10 cells/ml. You dilute this and adding 9 ml o
ID: 143258 • Letter: 2
Question
2. af cells at a concentration of 10 cells/ml. You dilute this and adding 9 ml of sterile medium. This gives you cells. You remove 0.1 ml from this diluted tube and add 9.9of bacte concentration of bacterial cells in each of the What is the ion tube 2. 3. Suppose you have 20 ul of an expensive enzym purchase more. The enzyme has a concentration of 1000 Units/ml. You are going to do an experiment that requires tubes with a concentration of 1 Unit/ml ot e and you cannot afford a enzyme and each tube will have 5 ml total volume. A) How much enzyme does each tube require? B) How many tubes can you prepare before you run out of enzyme?Explanation / Answer
2. Concentration of bacteria that broth contains: 105 cells/ml
Use dilution formula, C1V1=C2V2
Dilution tube 1
105 cells/ml * 1ml = X * 10ml
X= 104 cells/ml
Dilution tube 2
104 cells/ml* 0.1ml = X*10ml
X = 102 cells/ml
3. Uisng the same C1V1=C2V2 equation,
we can calculate the amount of the stock enzyme used.
the stock concentration= 1000U/ml
the final concentration = 1U/ml
final volume = 5ml
1000U/ml*X = 1U/ml*5ml
X = 0.005 ml or 5 micro litre
A) Thus each tube requires 5 microlitre of the enzyme
b) You can make 20/5 = 4, 4 tubes of enzyme dilution as we have 20 microlitres of stock enzyme
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