When a region of DNA that contains the genetic information for a protein is isol
ID: 143384 • Letter: W
Question
When a region of DNA that contains the genetic information for a protein is isolated from a bacterial cell and inserted into a eukaryotic cell in a proper position between a promoter and a terminator, the resulting cell usually produces the correct protein. But when the experiment is done in the reverse direction (eukaryotic DNA into a bacterial cell), the correct protein is often not produced What is a possible explanation for this? 8. What is a biological and/or evolutionary purpose for alternative splicing?Explanation / Answer
eukaryotic DNA contains introns and exons whereas prokaryotic DNA has only exonic sequences.
So when prokaryotic DNA is inserted into eukaryotic cell, the protein is flawlessely expressed
as only exons are present. When euaryotic DNA is inserted into bacteria, as there are
both introns and exons, there might be faults in expression.To avoid this we generally use
cDNA of eukaryotes to express proteins in prokaryotes.
Splicing refers to removal of introns and joining of exons to form a continuous coding sequence.
Alternative splicing involves skipping of one or more exons. This leads to production of
different mRNAs and proteins.So alternative splicing increases the number of transcriptome
or proteome without affecting the genome.
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