How would you do for A-D? Thanks 1. (8 points) During lab, you used a set of BSA

Question

How would you do for A-D? Thanks

1. (8 points) During lab, you used a set of BSA standards with known concentration by diluting a 5 mg/ml stock solution of BSA. Calculate the final concentration of BSA in the problems below using the formula where C1 and VI are the concentration and volume of the starting solution, and C2 and V2 are the concentration and volume of the final diluted solution. Include proper units and show your work for full credit. A. 300 ul of 5 mg/ml BSA is added to 700 ul TBS B. 50 ul of 1.5 mg/ml BSA is mixed with 450 ul H20 and 500 ul Bradford Reagent C. 10 ul of 1 mg/ml BSA is mixed with 990 ul TBS. D. 10 ul of 0.1 mg/ml BSA is mixed with 990 ul TBS. Page 1 of 3

Explanation / Answer

A. C1= 5, V1= 300, V2 = 300 +700, C2 =?

C1V1=C2V2

5 X 300 = C2 X 1000

C2 = 5 X 300 / 1000 = 1.5 mg/ml

B.

C1V1=C2V2

1.5 X 50 = C2 X 1000

C2 = 0.075 mg/ml

C.

C1V1 = C2V2

1 X 10 = C2 X 1000

C2 = 0.01 mg/ml

D.

C1V1 =C2V2

0.1 X 10 = C2 X 1000

C2 = 0.001 mg/ml

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