You and your lab partner want to determine if there are bacteriophage in your en

Question

You and your lab partner want to determine if there are bacteriophage in your environmental samples. You partner is attempting to isolate phage by the direct isolation method and you are using the enrichment procedure. What are the differences between these two procedures?

A) direct plating allows the phage in the environmental sample to infect the bacteria for several days, going through multiple rounds of infection, which may favor unique phage that do not compete as well with other bacteriophage in the environmental sample

B) enrichment allows the phage in the environmental sample to infect the bacteria for several days, going through multiple rounds of infection, which may favor unique phage that do not compete as well with other bacteriophage in the environmental sample

C) direct plating extracts the phage from the environmental sample in the absence of any bacteria, which may favor unique phage that do not compete as well with other bacteriophage in the environmental sample

D) enrichment plating extracts the phage from the environmental sample in the absence of any bacteria, which may favor unique phage that do not compete as well with other bacteriophage in the environmental sample

Explanation / Answer

Option D is the correct answer as enrichement media allow growth of specific type of sample and easily distinguish between other component.

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