QUESTION 8 Which of the following is false? O a The translocations associated wi

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QUESTION 8 Which of the following is false? O a The translocations associated with Burkitt's lymphoma always involve the c-myc gene on chromosome 8 b. Clinicians often assess the amplification and overexpression of relevant genes before selecting a course of therapy Techniques include iHC, which detects gene amplification, and FISH, which detects protein overexpression Genes that are frequently amplified in cancers include the epidermal growth factor receptor (EGFR, HER-2) d Microarrays can readily detect genes that show increase in copy number or increase in level of gene expression O c. QUESTION9 Which of the following is not a general mechanism involved in the conversion of proto-oncogenes to oncogenes? promoter methylation b. opoint mutation transiocation

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Question 8 :

false statement : clinicians always assess the amplification and overexpression of relevant genes before selecting a course of therapy.Techniques include IHC(Immunohistochemistry),which detects gene amplification and FISH,which detects protein over-expression.

Immunohistochemistry(IHC):involves the process of selectively imaging antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.Technique cannot be used to detect gene amplification.

FISH(Fluorescent in situ hybridization): molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity.Used to detect and localize the presence or absence of specific DNA sequences on chromosomes. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. FISH can also be used to detect and localize specific RNA targets (mRNA, lncRNA and miRNA) in cells, circulating tumor cells, and tissue samples. In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues. Cannot be used to detect protein over expression.

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