Question 11 RecA mechanism and properties: Which of the following statements is

Question

Question 11 RecA mechanism and properties: Which of the following statements is False? DNA isheld in form that is 1.5 times extended from normal B form (one turn per 18.6 nt). RecA aggregates into long filaments with ss- or ds-DNA. 6 RecA monomers per turn of filament which has a deep groove containing the DNA. There are 3 nucleotides per RecA monomer. The first step is for RecA to bind the double-stranded DNA. Then the single-stranded DNA is incorporated into the structure o Ina paranemic joint, the two strands lie side by side without intertwining.

Explanation / Answer

Answer 11 The false statement is 3 i.e The first step is for RecA to bind ds DNA. Then ss DNA is incorporated into the structure

Reason Whenever recombination between ssDNA and dsDNA occurs, first one to bind Rec A is ssDNA and not dsDNA

Answer 12 Correct option is 4 i.e. RecBCD (bacteria) and the 9 member complex containing Rad50 (yeast)

Reason Both have same function of end resection i.e. adding of long ssDNA chain on the 3' end during homologous recombination repair process.

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