l Vodafone Qatar 32% . 10:05 PM a mybb.qu.edu.qa 20-II A circular plasmid G has
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l Vodafone Qatar 32% . 10:05 PM a mybb.qu.edu.qa 20-II A circular plasmid G has a size of 5kb (5000 bp). The plasmid carries a gene of resistance to Tetracycline. Which experiments should you perform to prove that a bacterial strain carries this plasmid? Propose two different experiments: 1- 2- 10-II In a double stranded DNA, the Optic density measured at 260 nm is 0.8: What is the concentration of the DNA? 30-IV- In eukaryotic cells there are 3 families of sequences: Cite them and give an example of each of the 3 families: 1- 2-Explanation / Answer
answer 1
take all the bacterial strains and place in the antibiotic tetracyclines, when that time the entier bacteria will undergo death except the strain contraining tetracycline resistant plasmid.
Answer 2
here we are using an equation.
Concentration = absorbance * dilution factor * 50ug/ml
A260 of 1 = 50ug/ml of pure DNA.
Substitute the values and solve.
Answer 3
3 families of sequence are
# DNA sequence (example: junk sequence, Ori sequence, orf sequence, promoter sequences, gene)
# RNA sequences (example: mRNA, rRNA, tRNA) and
# Protein sequence.(example: transmembrane protein, TLR protein, G protein, )
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