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Given that root cells of the rml mutant are not abnormal in terms of size and sh

ID: 144909 • Letter: G

Question

Given that root cells of the rml mutant are not abnormal in terms of size and shape, speculate how the absence of a functional gene product (RML protein) in the mutant might affect cell division. In writing an answer to this question, start with the big picture first, considering whether the gene product that is mutated is an integral component of the division cycle operating throughout the whole plant, or perhaps regulates a specific division cycle event, and whether or not this affect is restricted to the root meristem and what can be inferred from the latter observation.

Cotyledons Cotyledons Seed Seed 8

Explanation / Answer

The gene product mutated only regulated the cell specific Divi Sion event and it is restricted to only the root meristem the mutant gene product is only produced during root development from post embryonic meristematic tissue and
proliferation of mutant embryo, shoot, and callus
cells indicates that the RML genes are not required for the
general cell division processes as are some of the cdc2 and
cyclin genes expressed in a11 proliferating cells.

The rml mutant phenotype is undetectable in the embryo but ap-pears immediately after germination, indicating that the

RML genes are required for cell proliferation during post embryonic root growth so there are specific and gene product of rml is formed only during this period of cell division

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