Step 1. Step 2. Step 3. Step 4. Step 5. Step 6. Step 7. Step 8. Step 9. Step 10.
ID: 145080 • Letter: S
Question
Step 1.
Step 2.
Step 3.
Step 4.
Step 5.
Step 6.
Step 7.
Step 8.
Step 9.
Step 10.
Step 11.
Step 12.
Step 13.
Step 14.
Step 15.
Step 16.
Don appropriate personal protective equipment (PPE).
Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10-5 cumulative dilution.
Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10-7 cumulative dilution.
Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10-4.
Allow the inocula to soak into the plates before proceeding.
Aseptically transfer 0.1 mL from dilution tube 2 to plate A1. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10-6 cumulative dilution.
Obtain eight plates, organize them int four pairs and label them A1, A2, B1, B2, etc.
Keep accurate records in your lab notebook.
Clean up the lab benches, doff PPE, and wash hands.
Repeate the procedure with plate A2 and label both plates "10-5mL of original sample," or simply "10-5"
Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10-4 dilution, but the plate is a 10-5 dilution!!
Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
Incubate the plates at 35 for 24 to 48 hours.
Obtain five dilution tubes and label them 1–5, respectively. Make sure they remain covered until needed.
Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10-2 dilution
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 1.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 2.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 3.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 4.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 5.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 6.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 7.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 8.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 9.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 10.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 11.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 12.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 13.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 14.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 15.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.Step 16.
A.Don appropriate personal protective equipment (PPE).
B.Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10-5 cumulative dilution.
C.Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10-7 cumulative dilution.
D.Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10-4.
E.Allow the inocula to soak into the plates before proceeding.
F.Aseptically transfer 0.1 mL from dilution tube 2 to plate A1. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
G.Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10-6 cumulative dilution.
H.Obtain eight plates, organize them int four pairs and label them A1, A2, B1, B2, etc.
I.Keep accurate records in your lab notebook.
Clean up the lab benches, doff PPE, and wash hands.
J.Repeate the procedure with plate A2 and label both plates "10-5mL of original sample," or simply "10-5"
Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10-4 dilution, but the plate is a 10-5 dilution!!
K.Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
L.Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
M.Incubate the plates at 35 for 24 to 48 hours.
N.Obtain five dilution tubes and label them 1–5, respectively. Make sure they remain covered until needed.
O.Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
P.Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10-2 dilution
Explanation / Answer
1.Don appropriate personal protective equipment (PPE).
2.Obtain five dilution tubes and label them 1–5, respectively. Make sure they remain covered until needed.
3.Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
4.Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10-2 dilution.
5.Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10-4.
6.Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10-5cumulative dilution.
7.Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10-6cumulative dilution.
8.Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10-7cumulative dilution.
9.Obtain eight plates, organize them int four pairs and label them A1, A2, B1, B2, etc.
10.Aseptically transfer 0.1 mL from dilution tube 2 to plate A1. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
11. Repeate the procedure with plate A2 and label both plates "10-5mL of original sample," or simply "10-5"
Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10-4 dilution, but the plate is a 10-5 dilution!!
12.Allow the inocula to soak into the plates before proceeding.
13.Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
14.Incubate the plates at 35 for 24 to 48 hours.
15.Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
16.Keep accurate records in your lab notebook.
Clean up the lab benches, doff PPE, and wash hands.
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