5. A mutant screen is carried out in Neurospora crassa, searching for mutants th

Question

5. A mutant screen is carried out in Neurospora crassa, searching for mutants that are u synthesize the amino acid serine. Ten mutants are isolated and tested for ce combinations using heterokaryon to grow in minimal medium and indicates inability to grow in minimal medium heterokaryons. The results are shown below, in which + indicates the ability of the 10 A. Write the numbers of the mutants that make up the 5 complementation groups (classes) that represent five different genes in the serine biosynthetic pathway based on the table above. Hint: mutants that do not have a complement most likely represent unique mutations. b. 10 mutants were found to represent 5 classes of mutants through complementation tests, In determine the identity of the mutant enzyme present in each class, each of the 5 classes were grown c minimal media supplemented with serine precursors abbreviated A, B, C, D and E. Below is a tab summarizing the results. Growth in the media is represented by a + and a - means no growth A_B | C | D | E | serine Class 1 Class 2+ Class 3 Class 4 Class 5 t Assuming one gene encodes one enzyme and that the pathway is linear, diagram the possible pathway showing the positions of the intermediates A E and serine. Use a right arrow to indicate each enzymac step in the pathway, and label each arrow with the Class number that represents the mutated gene blocks the enzymatic step

Explanation / Answer

Answer

Complementation group

1,4 and 1,6 = 1,4,6
5,7 and 5,10 = 5,7,10
8,9
3
2

b. B-D-A-C-E-Serine

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