3. Cost analysis of biochemical assay problem - how much does it cost to run a s

Question

3. Cost analysis of biochemical assay problem - how much does it cost to run a series of biochemical assays in 384 well plates at a cost of $0.50/well. The following assays were run: a large library was screened in one assay (250,000 drugs, one drug per well). Only 16 of the best were progressed to a second assay and those 16 were each run at 12 different concentrations in duplicate (x2 for each). Total cost? What is the number of 384 well plates used? What detection technique is used to screen biochemical assay?

Explanation / Answer

Cost for 384 well plates will be 192$..

Out of this , 16 wells were progressed for further assay at 12 different concentrations in duplicate .=> 16x2x12= 384. Total cost will be 192+192= 384$ ..

16 plates were used for second assay .. in duplicate means x2 as per given .. so 32 plates ..

The technique used is spectrophotometric measurements and gel staining .

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