The composition of solutions as reported in scientific journals is the final con

Question

The composition of solutions as reported in scientific journals is the final concentrations of the calculations necessary to create a "recipe. Concept #2: the phi only. If you wish to prepare the solution in your own lab, you need to do An experiment you wish to reproduce lists one of the buffers used as "Tris-buffered saline CIBS, 150 mM NaCI, 20 mM Tris, pH 7.5)." Describe how to prepare IL of TBS, pH7.5. The formula weights of NaCl and Tris are 58.44 and 121.14, respectively. Note that solutions of Tris have a very basic pH (-11) and may require significant volumes of HCI to adjust the pH to physiologic levels. You need to describe how to allow for that added volume when you write your "recipe." 4. You decided to prepare the TBS described above as a 10X stock solution that can be diluted with ddH2O to a 1X solution "on demand." Describe how to prepare 100 mL of 10X TBS. Report the concentration of the individual components in the 10X stock, the final pH, how much of both of the solids to combine, and how to allow for the volume required to adjust the pH.

Explanation / Answer

3. Recipe for 1 X TBS buffer a solution that contains 0.05M Tris, 150mM NaCl at pH 7.6.

Preparation of buffer:

4. For 100ml of 10X TBS you will require,

Tris Base= 2.42 g

NaCl = 8g

make the volume upto 100ml. This is 10X TBS solution.

We can make 1x solution from 10X TBS by using formula , C1V1=C2V2

C1= the concentration of undiluted solution

V1= volume of undiluted solution added to d.H2O

C2= desired concentration

V2= desired volume.

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