153 QUESTIONS FOR LAB REPORT 3 (turn in the answers typed, on a separate sheet o
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153 QUESTIONS FOR LAB REPORT 3 (turn in the answers typed, on a separate sheet of paperl) What does the term template mean? transcription, and for translation? 1. What are the templates for DNA replication, for How does the process of transcription differ from translation? To answer this question, construct a table similar to that shown in the transcription section of the lab report. Include as many differences as you can. 2. 3. The term that means the "3 nucleotides (bases) that code for one amino acid" depends on whether you are referring to DNA, mRNA or tRNA. In DNA, it is called a DNAIn mRNA, it is called a- . In tRNA, it is called an Suppose that there were mutations during the DNA replication and the coding strand ended up CCTCCGCATCCA instead of CGTCCACGTCCA. How would this affect the sequence of amino 4. acids? Show and compare the specific amino acid sequences for each peptide. Did all the mutations make a difference? Explain. You have a polypeptide chain that begins with the following amino acid sequence: tyrosine- asparagine-tryptophan. Make a DNA for this part of the polypeptide. Show the base sequence for both the coding and noncoding strands. Is there more than one possibility for your DNA?If so, show the coding and noncoding strands for all possibilities. 5.Explanation / Answer
1. The term template indicates the strand which is used for synthesis of their complimentray strand. In DNA replication, both strands are used as template as replication occurs in both direction by using both strands. In transcription, one strand of DNA is coding strand and other strand is noncoding strand. The noncoding strand is used as template for the synthesis of mRNA. In case of translation, the mRNA single strand is used as template which interct with their complimentary nucleotides on tRNA.
2. Transcription is the process of synthesis of mRNA from DNA while translation is the process of synthesis of protein from mRNA.
Transcription does not require ribosomes while translation requires ribosomes.
Transcription does not require tRNA while translation require tRNA.
The end product of transcription is mRNA while the end product of tr4anslation is protein.
3. Genetic code, Codon, and anticodon.
4. The coding strand before mutation encodes for amino acids: Pro-Pro-His-Pro. After mutation, the amino acis produced will be: Arg-Pro-Arg-Pro.
No, Not all the mutation make a difference as one amino acid may be encoded by more than one genetic code. For example, here CCG has been mutated into CCA but still the amino acid is same that is proline in both cases because both codes for same amino acid that is proline.
5. Tyrosine is encoded by UAA or UAC, Aspargine is coded by AAU or AAC, Tryptophan is encoded by UGG. Thus, the possible DNA nucleotide sequence will be as follows: TAAAATUGG and TACAACUGG
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