Help With Genetics (DNA extraction) Problem.. I\'m not sure if what I have is ri

Question

Help With Genetics (DNA extraction) Problem.. I'm not sure if what I have is right and need help on the rest.

2. It is important to keep track of the location of the DNA during the extraction process. For each of the steps below, where is the DNA extract located: a) After centrifuging down the neutralized fish tissue lysate: supernatant of precipitate b) After centrifuging the supernatant through the spin column precipitate supernatant or After centrifuging the wash solution through the spin column: supernatant or precipitate c) d) After centrifuging the elution solution through the column supernatant or precipitate

Explanation / Answer

a. Supernatant- it is the clear liquid which lies over precipitate.

b.precipitate

DNA remains in the column. The centrifuge forces the binding solution through a silica gel membrane that is inside the spin column. This removes any remaining impurities from the membrane, leaving only the nucleic acid bound to the silica gel.

c.precipitate

DNA will remain in the column.

d.supernatant

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