Translation MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFE SFGDLSTPDAVMGNPKVKAHGKK

Question

Translation MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFE SFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPE NFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

Note--there are 2052 total nucleotides in this sequence. They are arranged in blocks of 10, with six blocks of 10 on each line. The number on the left of each line = the number of the first nucleotide in that line. There is no biological significance to grouping the bases this way--it is merely done to make it easier for you to find a particular base in the sequence.

SEQUENCE (5’ to 3’ sequence of the coding strand)

1 ccctgtggag ccacacccta gggttggcca atctactccc aggagcaggg agggcaggag

61 ccagggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctgacac

121 aactgtgttc actagcaacc tcaaacagac accatggtgc acctgactcc tgaggagaag

181 tctgccgtta ctgccctgtg gggcaaggtg aacgtggatg aagttggtgg tgaggccctg

241 ggcaggttgg tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcatgt

301 ggagacagag aagactcttg ggtttctgat aggcactgac tctctctgcc tattggtcta

361 ttttcccacc cttaggctgc tggtggtcta cccttggacc cagaggttct ttgagtcctt

421 tggggatctg tccactcctg atgctgttat gggcaaccct aaggtgaagg ctcatggcaa

481 gaaagtgctc ggtgccttta gtgatggcct ggctcacctg gacaacctca agggcacctt

541 tgccacactg agtgagctgc actgtgacaa gctgcacgtg gatcctgaga acttcagggt

601 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca

661 taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa tgattgcatc

721 agtgtggaag tctcaggatc gttttagttt cttttatttg ctgttcataa caattgtttt

781 cttttgttta attcttgctt tctttttttt tcttctccgc aatttttact attatactta

841 atgccttaac attgtgtata acaaaaggaa atatctctga gatacattaa gtaacttaaa

901 aaaaaacttt acacagtctg cctagtacat tactatttgg aatatatgtg tgcttatttg

961 catattcata atctccctac tttattttct tttattttta attgatacat aatcattata

1021 catatttatg ggttaaagtg taatgtttta atatgtgtac acatattgac caaatcaggg

1081 taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt ttttgtttat

1141 cttatttcta atactttccc taatctcttt ctttcagggc aataatgata caatgtatca

1201 tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag gcaatagcaa

1261 tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag gtttcatatt

1321 gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg ggataaggct

1381 ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct cttatcttcc

1441 tcccacagct cctgggcaac gtgctggtct gtgtgctggc ccatcacttt ggcaaagaat

1501 tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct aatgccctgg

1561 cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag gttcctttgt

1621 tccctaagtc caactactaa actgggggat attatgaagg gccttgagca tctggattct

1681 gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt ctgaatattt

1741 tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg atgagctgtt

1801 caaaccttgg gaaaatacac tatatcttaa actccatgaa agaaggtgag gctgcaacca

1861 gctaatgcac attggcaaca gcccctgatg cctatgcctt attcatccct cagaaaagga

1921 ttcttgtaga ggcttgattt gcaggttaaa gttttgctat gctgtatttt acattactta

1981 ttgttttagc tgtcctcatg aatgtctttt cactacccat ttgcttatcc tgcatctctc

2041 tcagccttga ct

7. Codon 9 is an AAG codon; it causes lysine to be incorporated as the ninth amino acid in the polypeptide. Which would be more deleterious to the function of the protein, an AG change involving the first A in the codon (new codon = GAG) or an AG change involving the second A in the codon (new codon = AGG)? Why do you say this?

8. If you were to have a single nucleotide substitution in intron 2, would it be more deleterious to have that substitution occur at nucleotide 600, or at nucleotide 1200? Explain your answer.

Explanation / Answer

Answer 7 If codon AAG (Lysine) is replaced by GAG (Glutamic acid) or AGG (Arginine), replacement by Glutamic acid i.e. codon replacement of AAG by GAG will be more deleterious.

It is because lysine and arginine share certain properties like being basic, positively charged and polar in nature, while glutamic acid is acidic and negatively charged in nature which totally reversal of basic as welll as positively charged property of lysine. So, it will disrupt the interactions of side chain -NH2 group of lysine. This will not happen if replacement is by Arginine as it also contains side chain -NH2 group.

Answer 8 As introns are not translated to functional poplypeptides, they have a role in regulation of gene expression. Nucleotide 600 is nearer to Codon triplet tga (586-588) which in mRNA would be uga. This is a stop codon for the previous exon. So, this nucleotide 600 lies at just start of intron  and so will be more damaging as it will affect splicing process of mRNA. Codon 1200 is not having such a case so it is not that damaging.

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